Proliferating Cell Nuclear Antigen Labeling

Proliferating cell nuclear antigen (PCNA) can be used to label proliferating cells [26 (link)]. Double-staining immunofluorescence was carried out to determine the LC proliferation rate as previously described [17 (link)]. The slides were permeabilized with 0.1% Triton X-100. After primary antibody incubation, Alexa-conjugated secondary antibodies (Cell Signaling Technology, Boston, MA, USA) were followed to label CYP11A1 (green) and PCNA (red). The PCNA-labeling index was presented.

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Li H., Su M., Lin H., Li J., Wang S., Ye L., Li X, & Ge R. (2023). Patulin Stimulates Progenitor Leydig Cell Proliferation but Delays Its Differentiation in Male Rats during Prepuberty. Toxins, 15(9), 581.

Publication 2023

Alexa-conjugated secondary antibodies

Antibodies Antibody Cells Immunofluorescence Proliferating cell nuclear antigen Triton x 100

Corresponding Organization : Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

PCNA-labeling index

control variables

Concentration of Triton X-100 (0.1%) used for permeabilization
Incubation with primary antibody
Use of Alexa-conjugated secondary antibodies to label CYP11A1 (green) and PCNA (red)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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