Quantifying Serum BDNF Levels via ELISA

Levels of serum BDNF were quantified using a sandwich enzyme-linked immunosorbent assay for Human Free BDNF (Human BDNF Quantikine ELISA, R&D Systems, Minneapolis, MN) in accordance with the manufacturer’s instructions and our standard protocol (Bharani et al., 2017 (link), 2020 (link); Håkansson et al., 2017 (link)). This assay has an intra-assay and inter-assay variability of 5 and 9% respectively, and a minimum sensitivity of 20 pg/ml. Serum samples were diluted 20-fold in the dilution buffer provided in the assay kit and BDNF concentrations were determined via non-linear regression from the standard curves using GraphPad Prism version 8 (GraphPad Software, San Diego, CA).

Free full text: Click here

Grossberg A.N., Bettcher B.M., Gorgens K.A, & Ledreux A. (2021). Curiosity-Based Interventions Increase Everyday Functioning Score But Not Serum BDNF Levels in a Cohort of Healthy Older Adults. Frontiers in Aging, 2, 700838.

Publication 2021

Human BDNF Quantikine ELISA

Assay Buffer Dilution Elisa Human Human bdnf Prism Sensitivity Serum

Corresponding Organization : University of Denver

Other organizations : University of Colorado Anschutz Medical Campus

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Levels of serum BDNF

control variables

Standard protocol (Bharani et al., 2017, 2020; Håkansson et al., 2017)
Manufacturer's instructions for the Human BDNF Quantikine ELISA kit (R&D Systems)
Assay characteristics: intra-assay variability of 5%, inter-assay variability of 9%, and minimum sensitivity of 20 pg/ml
Serum samples diluted 20-fold in the dilution buffer provided in the assay kit

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!