All patients were diagnosed, treated, and received follow-up assessments at Hospital de Especialidades, Centro Médico Nacional Siglo XXI by the non-functioning adenoma clinic as part of the Endocrinology Service. The demographic, clinical, hormonal, and imaging characteristics of the patients are summarized in Table 1 .
After tumor extraction, the tissue was transported in the cold organ preservation medium, CUSTODIOL® (in mM: 15 NaCl, 9 KCl, 1 Alpha monopotassium ketoglutaric acid, 4 MgCl2 hexahydrate, 18 L-Histidine monohydrochloride monohydrate, 180 L-Histidine, 2 L-Tryptophan, 30 Mannitol, 0.015 CaCl2 dihydrate, 1000 mL vehicle g.s.). No more than 30 min elapsed between the surgery and the beginning of the experimental protocol. Pituitary tumors were divided into two sections with the aid of a scalpel; the first section was isolated to record the cell activity of intracellular calcium and the association of these cells with the vasculature. The second section was paraffin embedded and taken for routine characterization by immunohistochemistry using specific antibodies for the pituitary hormones (TSH, GH, PRL, FSH, LH, and ACTH) and transcription factors (NR5A1, POU1F1, and TBX19) [21 (link),39 (link)].
After tumor extraction, the tissue was transported in the cold organ preservation medium, CUSTODIOL® (in mM: 15 NaCl, 9 KCl, 1 Alpha monopotassium ketoglutaric acid, 4 MgCl2 hexahydrate, 18 L-Histidine monohydrochloride monohydrate, 180 L-Histidine, 2 L-Tryptophan, 30 Mannitol, 0.015 CaCl2 dihydrate, 1000 mL vehicle g.s.). No more than 30 min elapsed between the surgery and the beginning of the experimental protocol. Pituitary tumors were divided into two sections with the aid of a scalpel; the first section was isolated to record the cell activity of intracellular calcium and the association of these cells with the vasculature. The second section was paraffin embedded and taken for routine characterization by immunohistochemistry using specific antibodies for the pituitary hormones (TSH, GH, PRL, FSH, LH, and ACTH) and transcription factors (NR5A1, POU1F1, and TBX19) [21 (link),39 (link)].
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