The following duplexed siRNAs (predesigned Ambion Silencer Select) were obtained from Life Technologies: LIMK 1, 156129 and s69232; LIMK 2, 156133 and s69236; TPPP/p25, s91408 and s91407; nonmuscle cofilin, Cfl1, s63902; cdc42 siRNA, s63741; and negative control siRNA # 1. SMGs were transfected with 500 nM siRNA using RNAiFect (Qiagen, Valencia, CA) and cultured for 24–48 h, as we reported previously (Daley et al., 2009 (link))
Protocol detail
siRNA Knockdown of Cytoskeletal Regulators
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Cdc42
Cofilin
Limk 1
Limk 2
Sirna
Corresponding Organization :
Other organizations : Albany State University, University at Albany, State University of New York
Top 5 similar protocols
Variable analysis
independent variables
- LIMK 1 siRNA (156129 and s69232)
- LIMK 2 siRNA (156133 and s69236)
- TPPP/p25 siRNA (s91408 and s91407)
- Nonmuscle cofilin siRNA (Cfl1, s63902)
- Cdc42 siRNA (s63741)
- Negative control siRNA # 1
- Outcomes of silencing LIMK 1, LIMK 2, TPPP/p25, nonmuscle cofilin, and cdc42
- SMGs (Salivary Gland Cells) were cultured for 24-48 hours
- SiRNAs were transfected at 500 nM concentration using RNAiFect (Qiagen, Valencia, CA)
- Negative control siRNA # 1
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!