The patient‐derived xenograft GBM primary cell line TBD0220 derived from a female GBM patient with no history of radiation therapy or chemotherapy and underwent surgery at Hebei University‐affiliated hospital,[32 (link)
] and GBM cell line U87 was procured from the cell bank of the Chinese Academy of Sciences. TMZ‐resistant cells (TBD0220TR and U87TR) were derived from TBD0220 and U87 cells. All cells were maintained at 37 °C with 100% humidity and 5% CO2. Briefly, TBD0220 and U87 cells were exposed to a concentration of TMZ that was gradually increased from 1.25 mmol L−1 to a maximal dose of 160 mmol L−1. During the first induction phase, the culture media containing TMZ was replaced every 72 h. The concentration of TMZ was increased after maintaining the initial dose for 14 days and observing consistent cell growth. At each incremental step, the cultures were maintained for three to four weeks. The overall procedure took half a year.[33 (link)
] To maintain authenticity, all GBM cell lines were cryopreserved in liquid nitrogen. TBD0220 and TBD0220TR cells were cultivated in DMEM/F12 (Gibco) with 10% FBS, whereas U87 and U87TR cells were cultured in DMEM (Gibco) with 10% FBS. TMZ (#HY‐17364) and stiripentol (#HY‐103392) were acquired from MedChemExpress (China). TMZ and stiripentol were dissolved in DMSO (Sigma‐Aldrich, D2650, USA) to treat GBM cells.
] and GBM cell line U87 was procured from the cell bank of the Chinese Academy of Sciences. TMZ‐resistant cells (TBD0220TR and U87TR) were derived from TBD0220 and U87 cells. All cells were maintained at 37 °C with 100% humidity and 5% CO2. Briefly, TBD0220 and U87 cells were exposed to a concentration of TMZ that was gradually increased from 1.25 mmol L−1 to a maximal dose of 160 mmol L−1. During the first induction phase, the culture media containing TMZ was replaced every 72 h. The concentration of TMZ was increased after maintaining the initial dose for 14 days and observing consistent cell growth. At each incremental step, the cultures were maintained for three to four weeks. The overall procedure took half a year.[33 (link)
] To maintain authenticity, all GBM cell lines were cryopreserved in liquid nitrogen. TBD0220 and TBD0220TR cells were cultivated in DMEM/F12 (Gibco) with 10% FBS, whereas U87 and U87TR cells were cultured in DMEM (Gibco) with 10% FBS. TMZ (#HY‐17364) and stiripentol (#HY‐103392) were acquired from MedChemExpress (China). TMZ and stiripentol were dissolved in DMSO (Sigma‐Aldrich, D2650, USA) to treat GBM cells.
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