Determining Polymer Molecular Weights by SEC

Size exclusion chromatography was performed on a Dionex Ultimate 3000RSLC system (ThermoScientific, Sunnyvale USA) with RI detection. The columns were TOSOH TSKgel G3000PWXL-CP (7.8 x 300 mm, 7 μm) and TOSOH TSKgel G-oligoPW (7.8 x3 00 mm, 7 μm) coupled in series and operated isocratically at 1 mL/min with 0.1 M NaNO₃ as the mobile phase. Samples were dissolved in the mobile phase. The system was calibrated with DIN-pullulan standards with molecular masses of 6 kDa, 12 kDa, 22 kDa, 50 kDa and 110 kDa (PSS Polymer Standards Service, Mainz, Germany). Chromatography data were exported and treated by WinGPC Scientific v 6.20 software for estimation of average molecular weights, degree of polymerization, average molecular mass, and dispersity [21 (link)].

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Ganan M., Lorentzen S.B., Aam B.B., Eijsink V.G., Gaustad P, & Sørlie M. (2019). Antibiotic saving effect of combination therapy through synergistic interactions between well-characterized chito-oligosaccharides and commercial antifungals against medically relevant yeasts. PLoS ONE, 14(12), e0227098.

Publication 2019

Dionex Ultimate 3000RSLC system

Chromatography Polymer Polymerization Pullulan Size exclusion chromatography

Corresponding Organization : University of Oslo

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Variable analysis

independent variables

Size exclusion chromatography was performed on a Dionex Ultimate 3000RSLC system (ThermoScientific, Sunnyvale USA) with RI detection
The columns were TOSOH TSKgel G3000PWXL-CP (7.8 x 300 mm, 7 μm) and TOSOH TSKgel G-oligoPW (7.8 x3 00 mm, 7 μm) coupled in series
The system was operated isocratically at 1 mL/min with 0.1 M NaNO3 as the mobile phase
Samples were dissolved in the mobile phase

dependent variables

Average molecular weights
Degree of polymerization
Average molecular mass
Dispersity

control variables

The system was calibrated with DIN-pullulan standards with molecular masses of 6 kDa, 12 kDa, 22 kDa, 50 kDa and 110 kDa (PSS Polymer Standards Service, Mainz, Germany)

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