Metabolic Profiling in Murine Models

Whole body lean mass and fat mass were measured in avertin-anesthetized mice using dual energy X-ray absorptiometry. Plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) levels were determined enzymatically according to the manufacturer’s guidance (Applygen). For the glucose tolerance test, mice were fasted overnight for 16 h and then injected intraperitoneally with 1.5 g/kg body weight glucose. For the insulin tolerance test, mice were fasted for 6 h starting at 8 am and then injected with 1 IU/kg body weight insulin. Blood glucose levels were measured with a portable glucometer (Accu-Chek, Roche) at 0, 15, 30, 60, and 120 min after injection. Serum insulin levels were measured by Mouse Ultrasensitive Insulin ELISA kits (Alpco) as described before [17 (link)].

Free full text: Click here

Liu C., Dou Y., Zhang M., Han S., Hu S., Li Y., Yu Z., Liu Y., Liang X., Chen Z.J., Zhao H, & Zhang Y. (2024). High-fat and high-sucrose diet impairs female reproduction by altering ovarian transcriptomic and metabolic signatures. Journal of Translational Medicine, 22, 145.

Publication 2024

Accu-Chek Mouse Ultrasensitive Insulin ELISA kits

Corresponding Organization :

Other organizations : Shandong University, Shandong Women’s University

Top 5 similar protocols

Variable analysis

independent variables

Glucose dose (1.5 g/kg body weight)
Insulin dose (1 IU/kg body weight)

dependent variables

Whole body lean mass
Whole body fat mass
Plasma total cholesterol (TC)
Plasma low-density lipoprotein cholesterol (LDL-C)
Plasma high-density lipoprotein cholesterol (HDL-C)
Plasma triglyceride (TG) levels
Blood glucose levels at 0, 15, 30, 60, and 120 min
Serum insulin levels

control variables

Avertin anesthesia
Fasting duration (16 h for glucose tolerance test, 6 h for insulin tolerance test)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!