Peptide Synthesis and Purification

Rubiscolin-6 (YPLDLF) was synthesized by a solid-phase fluorenylmethyloxycarbonyl (Fmoc)-based strategy and an automated peptide synthesizer (Model Pioneer; Thermo Fisher Scientific), as previously described (23 (link)). The crude peptide was purified by reverse-phase high performance liquid chromatography (HPLC, Delta 600 HPLC System) using a Develosil ODSHG-5 column (2×25 cm; Nomura Chemical Co., Ltd.). High purity of the purified peptide was confirmed by analytical HPLC and MALDI-TOF MS analysis.

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Ataka K., Asakawa A, & Kato I. (2022). Rubiscolin-6 rapidly suppresses the postprandial motility of the gastric antrum and subsequently increases food intake via δ-opioid receptors in mice. Molecular Medicine Reports, 26(5), 340.

Publication 2022

Model Pioneer

Hplc Maldi ms Peptide Rubiscolin 6

Corresponding Organization :

Other organizations : Kobe Pharmaceutical University, Kagoshima University

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Variable analysis

independent variables

Synthesis method: Solid-phase fluorenylmethyloxycarbonyl (Fmoc)-based strategy
Peptide synthesizer: Automated peptide synthesizer (Model Pioneer; Thermo Fisher Scientific)

dependent variables

Purity of the purified peptide

control variables

Purification method: Reverse-phase high performance liquid chromatography (HPLC, Delta 600 HPLC System) using a Develosil ODSHG-5 column (2×25 cm; Nomura Chemical Co., Ltd.)

controls

Positive control: None specified
Negative control: None specified

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