Detailed Antibody Generation and Characterization

The custom-made antibodies used in this study were generated in rabbits against either selected regions or full-length proteins, as described previously (Wang et al. 1996 (link); Yang et al. 2009a (link); Sabath et al. 2013 (link)). Antibodies against Drosophila Lsm10 and Lsm11 (Liu et al. 2006 (link); Nizami et al. 2010 (link)) were kindly provided by Joe Gall (Carnegie Institution for Science). Anti-Flag M2 (F3165) and anti-SmB (S0698) mouse monoclonal antibodies were from Sigma.

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Skrajna A., Yang X.C., Bucholc K., Zhang J., Hall T.M., Dadlez M., Marzluff W.F, & Dominski Z. (2017). U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem–loop binding protein. RNA, 23(6), 938-951.

Publication 2017

Anti-Flag M2 (F3165)

Antibodies Drosophila Monoclonal antibodies Mouse Proteins Rabbits

Corresponding Organization :

Other organizations : University of North Carolina at Chapel Hill, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, National Institute of Environmental Health Sciences, National Institutes of Health

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Variable analysis

independent variables

Generating custom-made antibodies in rabbits against selected regions or full-length proteins

dependent variables

Not explicitly mentioned

control variables

Anti-Flag M2 (F3165) and anti-SmB (S0698) mouse monoclonal antibodies from Sigma

positive controls

Antibodies against Drosophila Lsm10 and Lsm11 provided by Joe Gall (Carnegie Institution for Science)

negative controls

Not specified

Annotations

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