Antibacterial Activity Assessment of PTMC Films

The activated Staphylococcus aureus was transferred to a nutrient solution and incubated at 37°C for 24 h. The bacteria were then appropriately diluted with this nutrient solution. Subsequently, the Staphylococcus aureus bacterial solution was removed with a sterile cotton swab and spread evenly over the nutrient agar plates. Three parallel samples were taken at each time point. The solution was then incubated at a constant temperature for 24 h. The diameter of the inhibition ring was measured using a vernier caliper with an accuracy of 0.1 mm, and the area without visible bacterial growth was visually observed as the edge of the inhibition ring. Staphylococcus aureus was also incubated with the control pure PTMC films.