Immunohistochemistry with Proteinase K

Immunohistochemistry was generally performed as described previously [51 (link)] with an additional tissue section digestion by Proteinase K (50 µg/mL; Sigma-Aldrich P 6556) for 10 min. Then the sections were stained using an immunohistochemical staining kit (DakoCytomation Carpinteria, CA, USA, LSAB+ system-HRP, K0679) and either the anti-EGFP-antibody from Abcam (ab290) in a 1:800 dilution or from cell signaling (255S) in a 1:500 dilution.

Free full text: Click here

Drummer C., Vogt E.J., Heistermann M., Roshani B., Becker T., Mätz-Rensing K., Kues W.A., Kügler S, & Behr R. (2021). Generation and Breeding of EGFP-Transgenic Marmoset Monkeys: Cell Chimerism and Implications for Disease Modeling. Cells, 10(3), 505.

Publication 2021

Proteinase K ab290

Anti antibody Digestion Dilution Immunohistochemistry Proteinase k Tissue

Corresponding Organization : German Centre for Cardiovascular Research

Other organizations : Friedrich-Loeffler-Institut, Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103, University of Göttingen

Top 5 similar protocols

Variable analysis

independent variables

Tissue section digestion by Proteinase K (50 µg/mL; Sigma-Aldrich P 6556) for 10 min

dependent variables

Immunohistochemical staining

control variables

Immunohistochemistry performed as described previously [51]

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!