We cultured human lung epithelial cells, BEAS-2B [29 (link)], in bronchial epithelial growth medium BulletKit at 37°C in a 5% CO2 atmosphere. The cells were divided into 10 groups (Table 2 ). We subcultured the cells at a density of 1 × 105/mL. After culturing the cells in the presence of 10 ng/mL TGF-β1 for 48 h, we transfected them with plasmid. The CRNDE NC-lncRNA, CRNDE si-lncRNA, NC mimic, miR-29a-3p mimic, oe-NC, and oe-MCL-1 shRNAs were supplied by GenePharma. We transferred BEAS-2B cells onto plates for transfections following the manufacturer's instructions.
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