Antibody Detection in Viral Infection

Western blot analysis was performed as previously described [36 (link)]. The primary antibodies and dilutions are: mouse anti-NP (ATCC, HB-65, 1:50), mouse anti-NS1 (Santa Cruz biotechnology, Santa Cruz, CA, USA, 1:1000), mouse anti-β-actin (Thermo Fisher Scientific, 1:3000), rabbit anti-FZD5 (ProSci, Poway, CA, USA, 1:1000).

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Yang X., Liang Y., Bamunuarachchi G., Xu Y., Vaddadi K., Pushparaj S., Xu D., Zhu Z., Blaha R., Huang C, & Liu L. (2020). miR-29a is a negative regulator of influenza virus infection through targeting frizzled 5 receptor. Archives of virology, 166(2), 363-373.

Publication 2020

mouse anti-NP mouse anti-NS1 mouse anti-β-actin

Actin Antibodies Dilutions Mouse Rabbit Western blot analysis

Corresponding Organization :

Other organizations : Oklahoma State University

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Variable analysis

independent variables

Mouse anti-NP (ATCC, HB-65, 1:50)
Mouse anti-NS1 (Santa Cruz biotechnology, Santa Cruz, CA, USA, 1:1000)
Rabbit anti-FZD5 (ProSci, Poway, CA, USA, 1:1000)

dependent variables

Western blot analysis

control variables

Mouse anti-β-actin (Thermo Fisher Scientific, 1:3000)

controls

Positive control: Not specified
Negative control: Not specified

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