Apoptotic analysis was performed as described [42 (link)]. CLL cells were cultured with or without NLCs or anti-Wnt5a antibody (MAB645; R&D Systems) (5 μg/mL) in RPMI culture-media, which were analyzed by Annexin V FITC Apoptosis Detection Kit I (BD Biosciences) and FACSCalibur Flow Cytometer (BD Biosciences). FlowJo (V10, FlowJo, OR, USA) was used for the analysis of apoptosis.
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