Immunohistochemical Staining Protocol

Primary antibodies used in this study include: Anti-βIII tubulin (abcam, ab107216, 1:1000 dilution)(Rinkevich et al. 2014 (link))), anti-ATF3 (C-19), (Santa Cruz SC-188, 1:1000 dilution, (Carozzi et al. 2013 (link))), anti-α-tubulin (Millipore, 04-1117, 1:100 dilution (Zhang et al. 2011 (link))), anti-acetylated tubulin(Cell Signal, 5335, 1:800 dilution, (Creppe et al. 2009 (link))), anti-EB1 (Millipore, AB6057, 1:500 dilution, (Vitre et al. 2008 (link))), anti-EB3 (Santa Cruz, SC-101475, 1:200 dilution, (Levy et al. 1994 (link))) and anti-phosphoneurofilament (Covance, SMI-31R, 1:2000 dilution, (Choi et al. 2008 (link))). Secondary antibodies used in this study included: Cy2-conjugated donkey anti-rabbit (711-225-152), Cy2-conjugated donkey anti rat (712-225-153), Cy3-conjugated donkey anti-chicken (703-165-155), and Cy5-conjugated donkey anti-mouse (715-175-151) (all purchased from Jackson Immunoresearch, (West Grove, PA)).

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Benbow S.J., Wozniak K.M., Kulesh B., Savage A., Slusher B.S., Littlefield B.A., Jordan M.A., Wilson L, & Feinstein S.C. (2017). Microtubule Targeting Agents Eribulin and Paclitaxel Differentially Affect Neuronal Cell Bodies in Chemotherapy Induced Peripheral Neuropathy. Neurotoxicity research, 32(1), 151-162.

Publication 2017

ab107216 SC-188 SMI-31R

Antibodies Cell Chicken Dilution Donkey Mouse Rabbit Tubulin α tubulin

Corresponding Organization :

Other organizations : University of California, Santa Barbara, Johns Hopkins University, Eisai (United States)

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Variable analysis

independent variables

Anti-βIII tubulin (abcam, ab107216, 1:1000 dilution)
Anti-ATF3 (C-19), (Santa Cruz SC-188, 1:1000 dilution)
Anti-α-tubulin (Millipore, 04-1117, 1:100 dilution)
Anti-acetylated tubulin(Cell Signal, 5335, 1:800 dilution)
Anti-EB1 (Millipore, AB6057, 1:500 dilution)
Anti-EB3 (Santa Cruz, SC-101475, 1:200 dilution)
Anti-phosphoneurofilament (Covance, SMI-31R, 1:2000 dilution)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

Annotations

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