A defrosted aliquot of bacteria was mixed with an equal volume of n-butanol (Merck) under stirring for 30 min at room temperature (RT). After centri-fugation at 13,000 g for 20 min, the aquatic phase was lyophilized, resuspended with chromatography start buffer (15% n-propanol in 0.1 M ammonium acetate, pH 4.7), and centrifuged at 45,000 g for 15 min. The supernatant was subjected to hydrophobic interaction chromatography (HIC) on octyl-Sepharose.
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Bacterial Lipid Extraction and Purification
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Ammonium acetate
Bacteria
Buffer
Butanol n
Chromatography
Hydrophobic interaction
Octyl sepharose
Propanol n
Corresponding Organization :
Other organizations : University of Konstanz
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Variable analysis
independent variables
- Mixing the defrosted aliquot of bacteria with an equal volume of n-butanol (Merck) under stirring for 30 min at room temperature (RT)
- Lyophilization of the aquatic phase
- Resuspension of the lyophilized material with chromatography start buffer (15% n-propanol in 0.1 M ammonium acetate, pH 4.7)
- Centrifugation of the resuspended material at 45,000 g for 15 min
- Subjecting the supernatant to hydrophobic interaction chromatography (HIC) on octyl-Sepharose
- Centrifugation at 13,000 g for 20 min
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