HeLa cells stably expressing hABH21–10-YFP and YFP were seeded into 96-well plates (4,000 cells/well) and incubated for 3 h. Various doses of MMS (Acros Organics), BCNU (1,3-Bis(2-chloroethyl)-1-nitrosurea; Sigma-Aldrich), TMZ (4-methyl-5-oxo-2,3,4,6,8-pentazabicyclo [4.3.0] nona-2,7,9-triene-9-carboxamide; Sigma-Aldrich), and MMC (6-amino-1,1a,2,8,8a,8b-hexahydro-8-(hydroxymethyl)-8a-methoxy-5-methyl-azirino[2’,3′:3,4] pyrrolo[1,2-a]indole-4,7-dione carbamate; Sigma-Aldrich) were added to the wells. The cells were exposed continuously until harvest. MTT was added to the cells, the OD was measured at 570 nm, the mean from at least six wells was used to calculate cell survival, and the SD was smaller than the size of the dots. Data presented show growth from one representative experiment and has been reproduced at least two times.
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Cytotoxicity Assay with DNA-Damaging Agents
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Other organizations : Norwegian University of Science and Technology
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Variable analysis
independent variables
- Various doses of MMS (Acros Organics)
- Various doses of BCNU (1,3-Bis(2-chloroethyl)-1-nitrosurea; Sigma-Aldrich)
- Various doses of TMZ (4-methyl-5-oxo-2,3,4,6,8-pentazabicyclo [4.3.0] nona-2,7,9-triene-9-carboxamide; Sigma-Aldrich)
- Various doses of MMC (6-amino-1,1a,2,8,8a,8b-hexahydro-8-(hydroxymethyl)-8a-methoxy-5-methyl-azirino[2',3′:3,4] pyrrolo[1,2-a]indole-4,7-dione carbamate; Sigma-Aldrich)
- Cell survival
- HeLa cells stably expressing hABH21–10-YFP and YFP
- Cell seeding density (4,000 cells/well)
- Incubation time (3 h)
- MTT assay for measuring cell survival
- Measurement of OD at 570 nm
- Mean from at least six wells used to calculate cell survival
- Standard deviation smaller than the size of the dots
- No positive or negative controls were explicitly mentioned.
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