For the isolation of hMADS cells from young donors, adipose tissue was obtained with the informed consent of the parents as surgical scraps from surgical specimen of various surgeries, as approved by the Centre Hospitalier Universitaire de Nice Review Board. We modified a previous published protocol used to isolate adipocyte precursors from adipose tissue (23 ). In brief, 200 mg/ml adipose tissue was dissociated for 5–10 min in DMEM containing antibiotics (100 U/ml of penicillin and 100 μg/ml of streptomycin), 2 mg/ml collagenase, and 20 mg/ml bovine serum-albumin. The crude SVF was separated from the adipocyte fraction by low speed centrifugation (200 g, 10 min). The adipocyte fraction was discarded and cells from the pelleted SVF were seeded onto uncoated tissue culture plates (Greiner) at 1,000–3,500 cells/cm2 in low glucose DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (D. Dutschers) and antibiotics as described before. Fast-adherent cells, termed CA cells, were separated from slow-adherent cells, termed CS cells. CA and CS cells were expanded in the same culture medium as described before. After reaching 70% confluence, cells were dissociated (0.25% trypsin EDTA; Invitrogen) and replated at 1,000–3,000 cells/cm2. Telomerase activity was determined by means of TeloTAGGG Telomerase PCR ElisaPLUS kit from Roche Diagnostic, according to the manufacturer's recommendations. SA β-galactosidase activity was determined according to Dimri et al. (24 (link)).