Isolation of Purified Blood Cells

Purified blood cell populations were obtained from six male healthy blood donors (mean age 38±13.6 years), previously recruited within the MALF study [43] (link). The sampling was done between July and October 2010 at the Fridhemsplan Blood Bank, Stockholm. 450 mL of blood were collected from the median cubital vein into transfusion bags containing citrate phosphate dextrose (Terumo Corporation, Japan), stored at room temperature and processed within 24 hours.

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Reinius L.E., Acevedo N., Joerink M., Pershagen G., Dahlén S.E., Greco D., Söderhäll C., Scheynius A, & Kere J. (2012). Differential DNA Methylation in Purified Human Blood Cells: Implications for Cell Lineage and Studies on Disease Susceptibility. PLoS ONE, 7(7), e41361.

Publication 2012

Blood Blood cell Blood donors Citrate phosphate dextrose Male Populations Transfusion Vein

Corresponding Organization :

Other organizations : Karolinska Institutet, University of Helsinki, Science for Life Laboratory

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Age of male healthy blood donors (mean age 38±13.6 years)
Blood was collected from the median cubital vein into transfusion bags containing citrate phosphate dextrose
Blood samples were stored at room temperature and processed within 24 hours

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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