Cell Apoptosis and Cell Cycle Assays

For cell apoptosis assays, the fluorescein isothiocyanate (FITC)–Annexin V and propidium iodide (PI) Apoptosis Detection kit I (BD Pharmingen) was used as previously described [29 (link)]. For cell cycle analysis, PEL cell pellets were fixed in 70% ethanol, and incubated at 4°C overnight. Cell pellets were re-suspended in 0.5 mL of 0.05 mg/mL PI plus 0.2 mg/mL RNaseA and incubated at 37°C for 30 min. Cell cycle distribution was analyzed on a FACS Calibur 4-color flow cytometer (BD Bioscience).

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Lam B.Q., Dai L., Li L., Qiao J., Lin Z, & Qin Z. (2017). Molecular mechanisms of activating c-MET in KSHV+ primary effusion lymphoma. Oncotarget, 8(11), 18373-18380.

Publication 2017

PI) Apoptosis Detection kit I FACS Calibur 4-color flow cytometer

Annexin v Apoptosis Assays Cell Cell cycle Ethanol Fluorescein Isothiocyanate Pellets Propidium iodide

Corresponding Organization :

Other organizations : Louisiana Cancer Research Center, Louisiana State University Health Sciences Center New Orleans, Tongji University, Shanghai East Hospital, Tulane University

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Variable analysis

independent variables

Exposure to fluorescein isothiocyanate (FITC)–Annexin V and propidium iodide (PI) Apoptosis Detection kit I (BD Pharmingen)
Fixing cell pellets in 70% ethanol
Incubation with 0.05 mg/mL PI plus 0.2 mg/mL RNaseA

dependent variables

Cell apoptosis
Cell cycle distribution

control variables

Incubation conditions (4°C overnight, 37°C for 30 min)
Flow cytometry analysis on FACS Calibur 4-color flow cytometer (BD Bioscience)

controls

Positive control: Not specified
Negative control: Not specified

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