Spontaneously Hypertensive Rat Drug Treatments

Adult (4 month-old) male spontaneously hypertensive rats (SHR) and normotensive control Wistar-Kyoto (WKY) rats (Charles River Laboratories, San Diego, CA) were studied as described previously [10 (link)]. All animal procedures were performed in accordance with NIH guidelines (Guide for the Care and Use of Laboratory Animals, revised 2011) and protocols approved by the Institutional Animal Care and Use Committee of Loma Linda University. For in vivo drug treatments, Y-27632 (0.3 mg/kg/h, ApexBio Technology, TX), CCG-100602 (7.5 mg/kg/d, ApexBio Technology, TX) or vehicle control (DMSO, Sigma-Aldrich) were continuously administered for 2 weeks by Alzet osmotic minipumps (Model 2ML2, DURECT, CA), implanted subcutaneously in rats under anesthesia with 2% isoflurane (JD Medical, AZ) [10 (link)].