Lung Cancer Cell Lines for Tumor Formation

Human lung cancer cell lines (CL1-0, F4, Bm7, Bm7brmx2, A549, and H1299) were used in this study as previously described16 (link). Cell lines were cultured within 6 months from the established cell bank to keep their ability to form lung cancer tumors in SCID mice. All cell lines were clean from Mycoplasma contamination. The following antibodies were used in western blots: anti-CDCP1 (ab1377, Abcam, Cambridge, MA, USA), anti-elongation factor 1 α (EF1α, #05-235, Millipore, Billerica, MA, USA), anti-ADAM9 (MAB939, R&D Systems, Minneapolis, MN, USA), and anti-ADAM9 (#2099, Cell Signaling, Danvers, MA, USA).