Sialidase-treated Chicken red blood cells (CRBCs) were prepared as previously described18 (link) with modified. CRBCs were washed and diluted to 10% (vol/vol) in PBS. 0.1 ml CRBCs were incubated with 10 μl (50 U/ml) of α2,3 –sialidase (Takara, Japan) for 15 min at 37 °C, washed three times in PBS, and re-suspended in PBS.
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