Frozen sections were fixed in methanol, blocked with albumin (Sigma, St. Louis, MO), and incubated with primary antibodies for 1 hour at 25°C (anti-dystrophin antibody, 1:400 dilution, Abcam, Cambridge, MA, USA; anti-PDK4 antibody 1:500 dilution). Immunostaining was performed using standard procedures as we described previously [14 (link)].
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