Antibody-based Analysis of UPR Pathways

Antibodies against the following proteins were used in this study: Bip (3177 s, Cell Signaling Technology [CST]), eIF2α (5324P, CST), Phospho-eIF2α (3398P, CST), SAPK/JNK (9252S, CST), Phospho-SAPK/JNK (9255S, CST), Caspase-12(2202S, CST), Cleaved Caspase-3 (9661S, CST), Cleaved PARP (9541S, CST), Phospho-EGFR and EGFR (11862S, CST), GAPDH (60,004–1-Ig, Proteintech). PE anti-human EGFR Antibody (352,904, Biolegend). The kinase inhibitor library was purchased from Shanghai Topscience Co., Ltd (Shanghai, China). Afatinib (HY-10261) were purchased from MCE.

Free full text: Click here

Wang G., Cao J., Gui M., Huang P., Zhang L., Qi R., Chen R., Lin L., Han Q., Lin Y., Chen T., He P., Ma J., Fu R., Hong J., Wu Q., Yu H., Chen J., Huang C., Zhang T., Yuan Q., Zhang J., Chen Y, & Xia N. (2023). The potential of swine pseudorabies virus attenuated vaccine for oncolytic therapy against malignant tumors. Journal of Experimental & Clinical Cancer Research : CR, 42, 284.

Publication 2023

GAPDH (60,004–1-Ig,

Afatinib Anti antibody Antibodies Caspase 12 Caspase 3 Egfr Gapdh Human Kinase Library Proteins

Corresponding Organization :

Other organizations : Xiamen University

Top 5 similar protocols

Variable analysis

independent variables

Kinase inhibitor library purchased from Shanghai Topscience Co., Ltd (Shanghai, China)
Afatinib (HY-10261) purchased from MCE

dependent variables

Bip (3177 s, Cell Signaling Technology [CST])
EIF2α (5324P, CST)
Phospho-eIF2α (3398P, CST)
SAPK/JNK (9252S, CST)
Phospho-SAPK/JNK (9255S, CST)
Caspase-12(2202S, CST)
Cleaved Caspase-3 (9661S, CST)
Cleaved PARP (9541S, CST)
Phospho-EGFR and EGFR (11862S, CST)
PE anti-human EGFR Antibody (352,904, Biolegend)

control variables

GAPDH (60,004–1-Ig, Proteintech)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!