Monocyte Phenotyping by Flow Cytometry

Flow cytometric analysis of monocytes was performed as previously described (11 (link)). To this end, cells were stained with the following monoclonal antibodies (BioLegend, Uithoorn, The Netherlands) in PBS supplemented with 0.1% BSA and 0.01% NaN₃: anti-human CD14-PE/Cy7 (clone: HCD14), anti-human CD16-APC/Cy7 (clone: 3G8), anti-human CD163-PE (clone GHI/61), anti-human CD192 (CCR2)-PerCP/Cy5.5 (clone: K036C2), and anti-human CX3CR1-FITC (clone: 2A9-1). Data were acquired on a FACS Canto II device (BD Bioscience) and analyzed using FlowJo® software (Tree Star, Ashland, OR).

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Fischer H.J., Finck T.L., Pellkofer H.L., Reichardt H.M, & Lühder F. (2019). Glucocorticoid Therapy of Multiple Sclerosis Patients Induces Anti-inflammatory Polarization and Increased Chemotaxis of Monocytes. Frontiers in Immunology, 10, 1200.

Publication 2019

anti-human CD14-PE/Cy7 anti-human CD163-PE clone FACS Canto II device

Cd163 Cells Clone Cy5 5 Device Fitc Flow cytometric Human Monoclonal antibodies Monocytes Nan3 Tree

Corresponding Organization : University of Göttingen

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Variable analysis

independent variables

Anti-human CD14-PE/Cy7 (clone: HCD14)
Anti-human CD16-APC/Cy7 (clone: 3G8)
Anti-human CD163-PE (clone GHI/61)
Anti-human CD192 (CCR2)-PerCP/Cy5.5 (clone: K036C2)
Anti-human CX3CR1-FITC (clone: 2A9-1)

dependent variables

Flow cytometric analysis of monocytes

control variables

PBS supplemented with 0.1% BSA and 0.01% NaN3

controls

No positive or negative controls were explicitly mentioned.

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