Measuring Lipid Absorption and CM Secretion

Lipid absorption and subsequent CM secretion were determined as previously described [25 (link)] with minor modifications. Briefly, 4-h fasted mice were injected with 500 mg/kg poloxamer-P407 (Sigma-Aldrich, St. Louis, MO) and 30 min post-injection, gavaged with 100 μl corn oil containing 2 μCi [³H]-triolein (Perkin Elmer, Waltham, MA), 0.5 μCi [¹⁴C]-cholesterol (ARC Inc, St. Louis, MO), and 0.25% cholesterol. Radioactivity in plasma was measured 1, 2, 3, and 4 h after gavage by liquid scintillation counting.

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Bianco V., Korbelius M., Vujic N., Akhmetshina A., Amor M., Kolb D., Pirchheim A., Bradic I., Kuentzel K.B., Buerger M., Schauer S., Phan H.T., Bulfon D., Hoefler G., Zimmermann R, & Kratky D. (2023). Impact of (intestinal) LAL deficiency on lipid metabolism and macrophage infiltration. Molecular Metabolism, 73, 101737.

Publication 2023

poloxamer-P407 [3H]-triolein

Cholesterol Corn oil Gavage Lipid Mice Plasma Poloxamer Radioactivity Secretion Triolein

Corresponding Organization : BioTechMed-Graz

Other organizations : University of Graz

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Variable analysis

independent variables

Poloxamer-P407 (500 mg/kg) injection
Corn oil gavage (100 μl) containing 2 μCi [3H]-triolein, 0.5 μCi [14C]-cholesterol, and 0.25% cholesterol

dependent variables

Radioactivity in plasma measured at 1, 2, 3, and 4 h after gavage by liquid scintillation counting

control variables

Mice fasted for 4 h prior to the experiment

controls

Positive control: Not specified
Negative control: Not specified

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