Stereological Quantification of TH+ Cells

Stereological probes were applied using a Zeiss Imager M2 microscope (Carl Zeiss, Jena, Germany) equipped with StereoInvestigator software (MBF Bioscience, VT, United States) according to previously published methods (Corenblum et al., 2015 (link); Anandhan et al., 2021 (link)). Using the optical fractionator probe, cells were counted under the ×63 oil immersion objective by a blinded observer. TH+ cells were counted in sections 480 μm apart using a grid size of 170 × 100 μm and counting frame size of 75 × 75 μm. The Gundersen method for calculating the coefficient of error was used to estimate the accuracy of the optical fractionator results. Coefficients obtained were less than 0.1.

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Apostol C.R., Bernard K., Tanguturi P., Molnar G., Bartlett M.J., Szabò L., Liu C., Ortiz J.B., Saber M., Giordano K.R., Green T.R., Melvin J., Morrison H.W., Madhavan L., Rowe R.K., Streicher J.M., Heien M.L., Falk T, & Polt R. (2022). Design and Synthesis of Brain Penetrant Glycopeptide Analogues of PACAP With Neuroprotective Potential for Traumatic Brain Injury and Parkinsonism. Frontiers in drug discovery, 1, 818003.

Publication 2022

Zeiss Imager M2 microscope StereoInvestigator software

Cells Frame Immersion Microscope Results

Corresponding Organization : University of Arizona

Other organizations : Phoenix VA Health Care System, Barrow Neurological Institute, Phoenix Children's Hospital, University of Bath, University of Colorado Boulder

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Variable analysis

independent variables

Stereological probes

dependent variables

Number of TH+ cells counted

control variables

Sections 480 μm apart
Grid size of 170 × 100 μm
Counting frame size of 75 × 75 μm
Gundersen method for calculating the coefficient of error
Coefficients obtained were less than 0.1

controls

Blinded observer

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