Antibody Use in Western Blot and ChIP Assays

The Arl8a/Arl8b antibody was a generous gift from Dr. Michael Brenner (Harvard) and was used at a 1:500 dilution for immunoblot [32 (link)]. The Zeb1 antibody (Cell Signaling Technologies, Beverly, MA) was used at 1:500. The LAMP-1 (H4A3) antibody was used at 1:200 (Developmental Studies Hybridoma Bank at Iowa State University). Transforming growth factor beta (Sigma Aldrich, St. Louis, MO) was used at 5 ng/mL. Anti-tubulin was acquired from NeoMarkers (Fremont, CA) and used at 1:20,000. Anti-actin was obtained from Sigma-Aldrich and used at 1:5,000. E-cadherin and cathepsin B antibodies (Santa Cruz Biotechnology, Dallas, TX) were used at 1:1,000. The cathepsin D antibody was obtained from Calbiochem (Billerica, MA) and used at 1:1,000. NHE1, vimentin, cathepsin L, and β-catenin antibodies were purchased from BD Biosciences (San Jose, CA) and were used at 1:1,000. Secondary antibodies include Dylight 594 donkey anti-mouse (1:200) (Jackson ImmunoResearch, West Grove, PA) and HRP-conjugated anti-mouse and anti-rabbit (1:5,000) (GE Healthcare, Pittsburgh, PA). Phalloidin 488 and 633 (Invitrogen, Carlsbad, CA) were used at 1:200. Primary antibodies used for ChIP were purchased from Santa Cruz Biotechnology: Mouse IgG (CS-2025) and Zeb1 (H-102).