Induction and Evaluation of CIA in Mice

The induction of CIA was described previously [58 (link)]. Briefly, 6 week-old DBA/1 J male mice (Shanghai SLAC Laboratory Animal Co.,Ltd) were immunized at the base of the tail with 200 µg bovine CII (Chondrex, USA) emulsified with CFA (Chondrex, USA), and boosted by immunization on day 21 in the same manner. The animal experiments were performed in accordance with the guidelines approved by Institutional Animal Care and Use Committee of Nanjing Medical University. The arthritis of mice was optically evaluated by using laser speckle imaging equipment (moorFLPI V5.0, Moor Instruments Ltd, UK).