Immunohistochemical Analysis of Steroid Receptors

The primary antibodies used in the present study included rabbit polyclonal anti-AR (sc-816, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-ERα (sc-542, Santa Cruz Biotechnology), rabbit polyclonal anti-ERβ (sc-8974, Santa Cruz Biotechnology), rabbit polyclonal anti-P450arom (derived from human Aromatase C-terminus, sc-30086, Santa Cruz Biotechnology). The dilution ranges of AR, ERα, ERβ and Aromatase antibodies for immunohistochemistry were all 1:500. The specificity of AR, ERα, ERβ and P450Arom antibodies have been described in our previous studies on WGS.^{14 (link),24 (link)} The immunohistochemistry kits with the secondary antibody of goat anti-rabbit was applied corresponding with the primary antisera.

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Li Q., Zhang F., Zhang S., Sheng X., Han Y., Yuan Z, & Weng Q. (2015). Seasonal Expression of Androgen Receptor, Aromatase, and Estrogen Receptor Alpha and Beta in the Testis of the Wild Ground Squirrel (Citellus Dauricus Brandt). European Journal of Histochemistry : EJH, 59(1), 2456.

Publication 2015

anti-AR (sc-816, sc-542, sc-30086

Antibodies Antibody Antisera Dilution Goat Human Immunohistochemistry P450arom Rabbit

Corresponding Organization :

Other organizations : Beijing Forestry University

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Variable analysis

independent variables

Rabbit polyclonal anti-AR (sc-816, Santa Cruz Biotechnology, Santa Cruz, CA, USA)
Rabbit polyclonal anti-ERα (sc-542, Santa Cruz Biotechnology)
Rabbit polyclonal anti-ERβ (sc-8974, Santa Cruz Biotechnology)
Rabbit polyclonal anti-P450arom (derived from human Aromatase C-terminus, sc-30086, Santa Cruz Biotechnology)

dependent variables

Immunohistochemistry measurements

control variables

Dilution ranges of AR, ERα, ERβ and Aromatase antibodies (all 1:500)
Specificity of AR, ERα, ERβ and P450Arom antibodies described in previous studies
Immunohistochemistry kits with the secondary antibody of goat anti-rabbit

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