Labeling and Dilution of rITs

HA22 (21 (link)) and LMB-11 (25 (link)) were produced as described.
rITs were labeled with Alexa Fluor 647 Labeling Kit (Invitrogen) according to manufacturer’s instructions. rITs for in vivo assays were diluted in phosphate buffered saline (PBS).
Secondary antibodies were purchased from Santa Cruz (Dallas, TX), primary antibodies (MCL1, PARP, EF2, GAPDH) from Cell Signaling (Danvers, MA), flow cytometry antibodies and Annexin V-PE/7-AAD from Becton Dickinson (Franklin Lakes, NJ).

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Müller F., Cunningham T., Liu X.F., Wayne A.S, & Pastan I. (2016). Wide variability in the time required for immunotoxins to kill B lineage acute lymphoblastic leukemia cells: Implications for trial design. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(19), 4913-4922.

Publication 2016

Alexa Fluor 647 Labeling Kit MCL1 GAPDH Annexin V-PE/7-AAD

Alexa fluor 647 Annexin v Antibodies Assays Flow cytometry Gapdh Ha22 Lmb 11 Mcl1 Phosphate Rits Saline

Corresponding Organization : Center for Cancer Research

Other organizations : Children's Hospital of Los Angeles, Children's Center, University of Southern California

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Variable analysis

independent variables

HA22 (21)
LMB-11 (25)

dependent variables

In vivo assays

control variables

Phosphate buffered saline (PBS)
Secondary antibodies from Santa Cruz
Primary antibodies (MCL1, PARP, EF2, GAPDH) from Cell Signaling
Flow cytometry antibodies and Annexin V-PE/7-AAD from Becton Dickinson

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

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