Protein separation and immunoblotting of spheroids
Corresponding Organization : Amsterdam UMC Location VUmc
Other organizations : Gdańsk Medical University, University of Pisa
Variable analysis
- Samples containing 50 µg protein of lysed spheroids (day 6)
- Cells grown in monolayer
- Target detection for LDH-A and β-actin
- Mini-PROTEAN® TGXTM Precast gels (Bio-rad, United States) used for protein separation
- Antibodies used for target detection: LDH-A (47010, 1:1000, Abcam, United Kingdom), β-actin (4790, 1:1000, Cell signalling, United States), Secondary IRDye® 800CW (926-32230, 1:10 000, Li-COR Bioscience, United States)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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