Samples containing 50 µg protein of lysed spheroids (day 6) and cells grown in monolayer were separated on Mini-PROTEAN® TGXTM Precast gels (Bio-rad, United States) as described previously [19 (link)]. Antibodies used for target detection; LDH-A (47010, 1:1000, Abcam, United Kingdom), β-actin (4790, 1:1000, Cell signalling, United States), Secondary IRDye® 800CW (926-32230, 1:10 000, Li-COR Bioscience, United States).
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