Trastuzumab-resistant Breast Cancer Cell Lines

Parental and resistant cell lines of SKBR3 and BT474 from the American Type
Culture Collection (ATCC, Manassas, VA, USA) and the German Collection of
Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) were donated
by Dr Park, Professor of the Department of Medical Oncology, Korea
University.
SKBR3 and BT474 epithelial breast cancer cells were cultured in the presence
of 50 μg trastuzumab (Roche, Basel, Switzerland) for approximately 3 years
to develop resistant cells. Parental cells were cultured in parallel with
resistant cells without the addition of trastuzumab. Resistance was
confirmed by cell viability assay. trastuzumab significantly reduced the
growth of wild-type parental cells compared to trastuzumab-resistant
cells.^{9 (link)} SKBR3 cells were grown in Roswell Park Memorial
Institute medium 1640 medium (Sigma-Aldrich, St. Louis, MO, USA), and BT474
cells were grown in Dulbecco’s modified Eagle’s medium (Hyclone, Logan, UT,
USA) with 10% fetal bovine serum (Hyclone) at 37°C in 5%
CO₂.^{10 (link)}