CSF samples were obtained by lumbar puncture, immediately centrifugated at 1000× g at 4 °C for 10 min and aliquoted in polypropylene tubes of 1.5 mL and stored at −80 °C until further analysis, according to international guidelines [47 (link)]. AD CSF biomarker measurements were performed in the biochemistry unit of Lariboisière Hospital [36 (link)]. Aβ42, Aβ40, t-Tau and p-Tau were first measured by ELISA, using available kits (INNOTEST®; Fujirebio, Ghent, Belgium) until July 2018, according to the manufacturer’s instruction. Then, the concentration of these biomarkers was measured by the analyzer Cobas 601 with the electrochemiluminescent reagents Elecsys® (Roche Diagnostics GmbH, Germany). The CSF profiles of patients were classified in A+T+N+/A-T-N-/or intermediate according to cut-offs determined by the laboratory.
CSF levels of α-syn were measured using an ELISA kit (ADx Neuroscience ELISA Alphasynuclein (Ghent, Belgium) available via EUROIMMUN, Lübeck, Germany), validated for research for the quantification of human total α-syn in CSF [48 (link)].
CSF levels of α-syn were measured using an ELISA kit (ADx Neuroscience ELISA Alphasynuclein (Ghent, Belgium) available via EUROIMMUN, Lübeck, Germany), validated for research for the quantification of human total α-syn in CSF [48 (link)].
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