The Bax and Hsp70 protein expressions were evaluated in each formalin-fixed paraffin-embedded wounded tissue section, as previously described in detail.14 (link) Immunostaining was performed using the EGFR pharmDx™ kit (DakoC ytomation, Carpinteria, CA, USA), according to the manufacturer’s protocol. In brief, endogenous peroxidase activity was quenched using a peroxidase block. Tissue sections were then incubated with Bax (1:200, Cat: ab7977; Abcam, Cambridge, MA, USA) and Hsp70 (1:500, Cat: ab2787, Abcam) biotinylated primary antibody for 15 minutes followed by another 15 minutes of incubation with streptavidin–horseradish peroxidase. The sections were incubated with diaminobenzidine tetrahydrochloride for 5 minutes, and then counterstained with hematoxylin and 0.5% ammonia in water. The brown illustrations of samples under a light microscope demonstrated the positive findings.