Generating HER2+ Breast Cancer Cell Lines

HER2+ breast cancer cells were transduced with pLKO.1-puro based shRNA MISSION lentiviral particles for DVL2, shDVL2 (TRCN000033340, Sigma), and non-target shRNA control, NTC (SHC002V, Sigma). After 24 h of plating, cells were transduced with hexadimethrine bromide (Sigma, H9268) at a final concentration of 8 μg/ml, followed by addition of appropriate amounts of viral particles to the media. After 24 h, the media was replaced and 1 μg/ml of puromycin (Gibco, A11138-03) was added for selection. puromycin-containing media was replaced every 3–4 days until total selection was achieved [30 (link)]. Supplementary Fig. S1A and B respectively demonstrate the selection process of SKBR3 and BT474 clonal cell lines.