RPASMCs were generous gifts from Dr. Zeng Yan, and cultured as previously reported [45 (link)]. Cultured primary RPASMCs (≤4 passage) were starved for 12 h (h) and then treated with PDGF-BB (30 ng/ml, R&D system, Minneapolis, MN) for different time (0, 2, 4, 6, 12, 24 h). miRNA mimics (20nM) were transfected with K2 transfection reagent (Biontex, Planegg, Germany) in RPASMCs. HEK-293a, a cell line derived from human embryonic kidney, was purchased from American Type Culture Collection (Manassas, USA). The transfection of miRNA mimic and DNA plasmids into HEK-293 cells were performed with polyethylenimine (PEI, Geneups, Shenzhen, China).
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