This method is useful to identify rare heterozygous diploid spore colonies that could be confused with recombinants (Subheading 3.3.4 ). It is also useful for screening colonies from heterothallic strains to identify homothallic variants, or vice versa, and for screening homothallic colonies for mutants that produce few viable spores, as expected for mutants that make DSBs but do not repair them (“late” Rec mutants).
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Screening Heterozygous Diploid Spores
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Corresponding Organization : Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
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Variable analysis
independent variables
- Identifying rare heterozygous diploid spore colonies that could be confused with recombinants
- Screening colonies from heterothallic strains to identify homothallic variants, or vice versa
- Screening homothallic colonies for mutants that produce few viable spores, as expected for mutants that make DSBs but do not repair them ("late" Rec mutants)
- Identifying rare heterozygous diploid spore colonies
- Identifying homothallic variants from heterothallic strains, or vice versa
- Identifying mutants that produce few viable spores
- Control variables not explicitly mentioned
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