Quantifying Short and Branched Fatty Acids

Frozen stool samples were extracted for short chain and branched chain fatty acids (SCFA and BCFA, respectively) using acidified water (pH 2.5), as described previously [25 (link)]. Peak areas were normalized to the total signal and represented as a percentage of total SCFA. Commercial standards of acetic acid, propionic acid, iso butyric acid, butyric acid, iso valeric acid, valeric acid, caproic acid and heptanoic acid (Sigma, St. Louis, MO, USA) were used to confirm compound identities.

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Sheflin A.M., Borresen E.C., Wdowik M.J., Rao S., Brown R.J., Heuberger A.L., Broeckling C.D., Weir T.L, & Ryan E.P. (2015). Pilot Dietary Intervention with Heat-Stabilized Rice Bran Modulates Stool Microbiota and Metabolites in Healthy Adults. Nutrients, 7(2), 1282-1300.

Publication 2015

acetic acid propionic acid isobutyric acid butyric acid isovaleric acid valeric acid caproic acid heptanoic acid

Acetic acid Acid Butyric acid Caproic acid Fatty acids Frozen Isobutyric acid Isovaleric acid Propionic acid Stool Valeric acid

Corresponding Organization :

Other organizations : Colorado State University, University of Colorado Denver, University of Colorado Hospital

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Variable analysis

independent variables

Frozen stool samples

dependent variables

Short chain fatty acids (SCFA)
Branched chain fatty acids (BCFA)

control variables

Acidified water (pH 2.5)
Commercial standards of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, caproic acid and heptanoic acid

controls

Commercial standards of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, caproic acid and heptanoic acid were used to confirm compound identities.

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