Sexually Transmitted Infection Screening Protocol

First-void urine was collected and routinely tested for NG and CT (Aptima Combo 2; Hologic Inc, San Diego, CA). Samples with equivocal results were retested using the Aptima CT single assay (Hologic Inc) and for NG with a quantitative polymerase chain reaction targeting opa genes.^{17 (link)} Urine samples were tested for MG using transcription-mediated amplification (TMA) assays (Aptima, Hologic Inc). From all samples that tested positive for MG in the MG-TMA assay, DNA was extracted with isopropanol precipitation and subsequently tested for macrolide resistance using the MG-MRAM quantitative polymerase chain reaction to detect wild-type (MG-WT) or any mutations (MRAM) in the 23S rRNA gene at nucleotide positions 2058 and 2059 (Escherichia coli numbering).^{18 (link)}Mycoplasma genitalium and MRAM testing were done for study purposes only, and results were disclosed neither to the men nor to the healthcare professionals. A subset of samples was tested for Trichomonas vaginalis (TV) using TMA assays (Aptima, Hologic Inc).

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Braam J.F., van Dam A.P., Bruisten S.M., van Rooijen M.S., de Vries H.J., Schim van der Loeff M.F, & Vergunst C.E. (2021). Macrolide-Resistant Mycoplasma genitalium Impairs Clinical Improvement of Male Urethritis After Empirical Treatment. Sexually Transmitted Diseases, 49(5), 360-367.

Publication 2021

Aptima Combo 2 Aptima CT single assay Aptima

Assays Escherichia coli Genes Healthcare professionals Isopropanol Macrolide Mutations Mycoplasma genitalium Nucleotide Polymerase chain reaction Rrna gene Transcription Trichomonas vaginalis Urine Void urine

Corresponding Organization : Geriant

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Variable analysis

independent variables

NG (Neisseria gonorrhoeae) testing method
CT (Chlamydia trachomatis) testing method
MG (Mycoplasma genitalium) testing method
Macrolide resistance testing method

dependent variables

Presence/absence of NG, CT, MG, and macrolide resistance

control variables

First-void urine samples
Aptima Combo 2 assay for initial NG and CT testing
Aptima CT single assay and NG quantitative PCR for retesting samples with equivocal results
Aptima TMA assays for MG testing
Isopropanol precipitation for DNA extraction from MG-positive samples
MG-MRAM quantitative PCR for macrolide resistance detection

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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