Recombinant Expression of Mouse MG53 and Orai1

Using full-length mouse MG53 DNA (GenBank accession number: NM_001079932) as a template, GST-fused full-length MG53 or MG53 domains were constructed and were expressed in E. coli (DH5α), as previously described30 (link)44 (link). GFP-tagged full-length MG53 or PRY-SPRY was constructed using an eGFP vector, as previously described16 (link). HA-MG53 was constructed, as previously described23 (link). Orai1-myc was constructed by inserting Orai1 DNA (GenBank accession number: NM_175423.3) into the 5′ end of pcDNA3.1 A containing a myc-tag (Invitrogen, Waltham, MA, USA)23 (link).

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Ahn M.K., Lee K.J., Cai C., Huang M., Cho C.H., Ma J, & Lee E.H. (2016). Mitsugumin 53 regulates extracellular Ca2+ entry and intracellular Ca2+ release via Orai1 and RyR1 in skeletal muscle. Scientific Reports, 6, 36909.

Publication 2016

myc-tag

E coli Mouse Vector

Corresponding Organization :

Other organizations : Catholic University of Korea, Albany Medical Center Hospital, Seoul National University, The Ohio State University

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Variable analysis

independent variables

Full-length mouse MG53 DNA (GenBank accession number: NM_001079932)
GST-fused full-length MG53 or MG53 domains
GFP-tagged full-length MG53 or PRY-SPRY
HA-MG53
Orai1-myc

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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