Prostate Cancer Cell Lines Cultivation

Human prostate cancer cell line PacMetUT1 was isolated from the lymph node metastasis of a 57-year old prostate cancer patient at our university [49 (link)]. PC-3 and DU145 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). PacMetUT1 and DU145 cells were cultured in McCoy’s 5A medium supplemented with pyruvate, amino acids, nutrients and 10% fetal bovine serum (FBS), as described [50 (link)]. HEK293 FreeStyle cells (HEK293F, Invitrogen, Carlsbad, CA) were maintained in FreeStyle 293 Medium (Invitrogen, Carlsbad, CA) supplemented with 1% Penicillin/Streptomycin. Mink lung epithelial (Mv1Lu) cells were obtained from Prof. Dan Rifkin and were maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 10% FBS and 1% penicillin/streptomycin. PacMetUT1, DU145, and Mv1Lu cells were maintained at 37°C in a 5% CO₂ humidified incubator, while the HEK293F cells were maintained at 37°C in an 8% CO₂humidified incubator-shaker.