Characterization of Anti-OFD1 Antibodies

The anti-OFD1 and anti-Ofd1, were generated against the human full-length OFD1 protein (NM_003611) and a portion of the murine Ofd1 homologous protein (NM_177429 Aa 461–884), respectively, and were previously described^{60 (link)}. All other antibodies used in this study are commercially available and are listed below. From Santa Cruz Biotechnology: eIF3G (R-20 sc-16362), eIF3B (H-300 sc-28857), GAPDH (6C5 sc-32233), GH (sc-10364), VPS39 (D15 sc-104759), NET1 (H-70 sc50392), IgG mouse (sc-2025), IgG rabbit (sc-2027). From Cell Signaling Technology: eIF4E (9742), eIF4G (2498), phosphorylated rpS6 (Ser240/244, 5364), eIF4A1 (2490), phosphorylated 4E-BP1 (Thr37/46, 9459). From Sigma-Aldrich: VCL (clone hVIN-1 V9131), γtubulin (clone GTU-88 T6557) and acetylated tubulin (T6793). From Spring Bioscience GDI2 (D4-GDI E2430). References for antibodies’ specificity^{14 (link), 61 (link)}: for eIF4E and eIF4A1^{62 (link)}; for eIF3G^{63 (link), 64 (link)}; for eIF3B.

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Iaconis D., Monti M., Renda M., van Koppen A., Tammaro R., Chiaravalli M., Cozzolino F., Pignata P., Crina C., Pucci P., Boletta A., Belcastro V., Giles R.H., Surace E.M., Gallo S., Pende M, & Franco B. (2017). The centrosomal OFD1 protein interacts with the translation machinery and regulates the synthesis of specific targets. Scientific Reports, 7, 1224.

Publication 2017

acetylated tubulin (T6793).

4e bp1 A protein Antibodies Clone Eif4e Eif4g Gapdh Human ofd1 protein Mouse Ofd1 Rabbit Tubulin Vps39

Corresponding Organization : University of Naples Federico II

Other organizations : Telethon Institute Of Genetics And Medicine, University Medical Center Utrecht, University Hospital Heidelberg, San Raffaele University of Rome, Istituto Nazionale Genetica Molecolare, Institut Necker Enfants Malades, Inserm, Sorbonne Paris Cité, Université Paris Cité

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Variable analysis

independent variables

The anti-OFD1 and anti-Ofd1 antibodies were generated against the human full-length OFD1 protein (NM_003611) and a portion of the murine Ofd1 homologous protein (NM_177429 Aa 461–884), respectively.

dependent variables

Not explicitly mentioned.

control variables

All other antibodies used in this study are commercially available and listed, including eIF3G, eIF3B, GAPDH, GH, VPS39, NET1, IgG mouse, IgG rabbit, eIF4E, eIF4G, phosphorylated rpS6 (Ser240/244), eIF4A1, phosphorylated 4E-BP1 (Thr37/46), VCL, γtubulin, acetylated tubulin, and GDI2.

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

Annotations

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