Antibodies and Plasmids Used in Myogenesis Study

Antibodies used in this study were as follows: anti-DYKDDDDK (anti-Flag) antibody (Trans Genic, Kobe, Japan), anti-SMα−actin, anti-α−tubulin antibodies, and anti-Flag M2 affinity gel (Sigma, St. Louis, MO), anti-myoD and anti-myogenin antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), anti-myosin heavy chain (MHC) antibody (MF20) (Developmental Studies Hybridoma Bank), and Alexa 568-conjugated secondary antibody (Molecular Probes, Eugene, OR). Concerning a polyclonal anti-MRTF-A antibody, we described in detail previously [13 (link)]. Detail information about the plasmids used in this study was described elsewhere [8 (link), 22 (link)].

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Watanabe B., Minami S., Ishida H., Yoshioka R., Nakagawa Y., Morita T, & Hayashi K. (2015). Stereospecific Inhibitory Effects of CCG-1423 on the Cellular Events Mediated by Myocardin-Related Transcription Factor A. PLoS ONE, 10(8), e0136242.

Publication 2015

anti-SMα−actin anti-α−tubulin antibodies anti-Flag M2 affinity gel Alexa 568-conjugated secondary antibody

Actin Alexa 568 Anti antibodies Anti antibody Antibodies Antibody Genic Hybridoma Molecular probes Myogenin Myosin heavy chain Plasmids α tubulin

Corresponding Organization : Osaka University

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Variable analysis

independent variables

Anti-DYKDDDDK (anti-Flag) antibody
Anti-SMα−actin antibody
Anti-α−tubulin antibody
Anti-Flag M2 affinity gel
Anti-myoD antibody
Anti-myogenin antibody
Anti-myosin heavy chain (MHC) antibody (MF20)
Alexa 568-conjugated secondary antibody
Polyclonal anti-MRTF-A antibody
Plasmids used in this study

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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