Pharmacological Modulation of Corneal Wound Healing

Immediately post wounding, the mice were topically administered the selective β2-AR agonist isoepinephrine (5 µL, 0.05 molL⁻¹; Tocris Bioscience, UK), the selective β2-AR antagonist timolol (5 µL, 0.01 molL⁻¹; Tocris Bioscience, UK), the α1-AR antagonist tamsulosin (5 µL, 20 molL⁻¹; Tocris Bioscience, UK). The aforementioned drugs were dissolved in a balanced salt solution (BSS), and the BSS was administered as the vehicle for the control mice. In some experiments, the mice received i.p. injections of the chemokine receptor CXCR2 antagonist SB 225002 (Tocris, 1 molL⁻¹, using dimethyl sulfoxide as vehicle) to inhibit neutrophil trafficking 5 min before ATSE^{39 (link)}. The effect of isoepinephrine, timolol, tamsulosin, and SB 225002 on corneal wound healing without ATSE was documented in Supplementary Figure 8 as the control data.