BaF3 cells (DSMZ, #ACC 300) were grown in RPMI media (Lonza, Basel, Switzerland) supplemented with 10% FBS, 50 U/ml penicillin, 50 µg/ml streptomycin, and 10% WEHI conditioned supernatant. For stable expression of human ROR1, ROR2, and PTK7 (or their truncated variants) in BaF3, proteins were cloned into the pEF-IRES-P vector [14 (link)] and transfected using Nucleofection as previously described [3 (link)].
293T cells were cultured in DMEM media (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 50 U/ml penicillin, 50 µg/ml streptomycin. Transient transfection of 293T cells was done using TurboFect (Thermo Fisher Scientific) according to the manufacturer’s instructions.
293T cells were cultured in DMEM media (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 50 U/ml penicillin, 50 µg/ml streptomycin. Transient transfection of 293T cells was done using TurboFect (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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