Plasma Biomarker Profiling Protocol

Plasma samples were stored immediately at −20°C after obtention. Soluble granzyme A and B were measured by sandwich ELISA (eBioscience; LD 2 pg/ml) in plasma, in accordance with the manufacturer’s recommendations. Human tumour necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70, and IFN-γ were measured by cytometric-bead-array multiplex assay (BD Biosciences; LD 0.5 pg/mL). Aspartate transaminase (AST), alanine transaminase (ALT) and renal function were measured in plasma with spectrophotometry (Roche Diagnostics) as for previous study [16 (link)].

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de Jong H.K., Garcia-Laorden M.I., Hoogendijk A.J., Parry C.M., Maude R.R., Dondorp A.M., Faiz M.A., van der Poll T, & Wiersinga W.J. (2017). Expression of intra- and extracellular granzymes in patients with typhoid fever. PLoS Neglected Tropical Diseases, 11(7), e0005823.

Publication 2017

sandwich ELISA cytometric-bead-array multiplex assay

Alanine transaminase Aspartate transaminase Assay Diagnostics Elisa Granzyme a Human Ifn γ Interleukin 10 Interleukin 12p70 Interleukin 1β Interleukin 6 Interleukin 8 Plasma Renal Spectrophotometry Tumour necrosis factor α

Corresponding Organization : Child Health Research Foundation

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Soluble granzyme A
Soluble granzyme B
Human tumour necrosis factor (TNF)-α
Interleukin (IL)-1β
IL-10
IL-12p70
IFN-γ
Aspartate transaminase (AST)
Alanine transaminase (ALT)
Renal function

control variables

Plasma samples were stored immediately at −20°C after obtention

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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