X. laevis embryos were obtained, cultured and injected as described [1 (link), 3 (link)–5 (link)]. Experiments were carried out with relevant UK animal licence and ethical approvals. Capped mRNA for injection was prepared from the cDNA using the mMessage mMachine kit (Ambion), and purified using illustra ProbeQuant G-50 Micro Columns (GE Healthcare). RNA was purified and RT-PCR was performed as described [4 (link), 6 (link)].
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