Antibody Profiling of Subcellular Organelles

Primary antibodies were: DLL1 (rabbit, Santa Cruz, sC-9102), Alpha 1 Sodium Potassium ATPase (mouse, Abcam, ab7671), GM130 (mouse, BD Biosciences, 610823), KDEL (mouse, Abcam, ab1223), EEA1 (rabbit, Abcam, ab2900), Rab5 (mouse, Sigma, R7904), Rab6A (mouse, Sigma, WH0005870M1), Transferrin receptor (mouse, Zymed, 13-6800), Caveolin (rabbit, BD Biosciences, 610060), Rab11 (rabbit, Sigma, R5903), Pan-Cadherin (mouse, Sigma, C1821), β-Actin (mouse, MPBiomedicals, 69100). Monoclonal antibodies against DLL1 were described [34] (link). Secondary antibodies were: Donkey anti-rat-Alexa488, goat anti-rat-Alexa488, goat anti-mouse-Alexa555, goat anti-rabbit-Alexa488, goat anti-mouse-Alexa633, goat anti-rabbit-Alexa555 (Molecular probes, Invitrogen).

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Müller J., Rana N.A., Serth K., Kakuda S., Haltiwanger R.S, & Gossler A. (2014). O-fucosylation of the Notch Ligand mDLL1 by POFUT1 Is Dispensable for Ligand Function. PLoS ONE, 9(2), e88571.

Publication 2014

GM130 Pan-Cadherin β-Actin Donkey anti-rat-Alexa488 goat anti-rat-Alexa488 goat anti-mouse-Alexa555 goat anti-rabbit-Alexa488 goat anti-mouse-Alexa633 goat anti-rabbit-Alexa555

Actin Antibodies Cadherin Caveolin Donkey Goat Molecular probes Monoclonal antibodies Mouse Rabbit Sodium potassium atpase Transferrin receptor

Corresponding Organization :

Other organizations : Medizinische Hochschule Hannover, Stony Brook University

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Variable analysis

independent variables

Antibodies used for immunostaining: DLL1, Alpha 1 Sodium Potassium ATPase, GM130, KDEL, EEA1, Rab5, Rab6A, Transferrin receptor, Caveolin, Rab11, Pan-Cadherin, β-Actin

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Monoclonal antibodies against DLL1 described in [34]
Negative control: Not specified

Annotations

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